KMID : 1161420160190070654
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Journal of Medicinal Food 2016 Volume.19 No. 7 p.654 ~ p.662
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Aronia melanocarpa Concentrate Ameliorates Pro-Inflammatory Responses in HaCaT Keratinocytes and 12-O-Tetradecanoylphorbol-13-Acetate-Induced Ear Edema in Mice
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Goh Ah-Ra
Youn Gi-Soo Yoo Ki-Yeon Won Moo-Ho Han Sang-Zin Lim Soon-Sung Lee Keun-Wook Choi Soo-Young Park Jin-Seu
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Abstract
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Abnormal expression of pro-inflammatory mediators such as cell adhesion molecules and cytokines has been implicated in various inflammatory skin diseases, including atopic dermatitis. In this study, we investigated the anti-inflammatory activity of Aronia melanocarpa concentrate (AC) and its action mechanisms using in vivo and in vitro skin inflammation models. Topical application of AC on mouse ears significantly suppressed 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema formation, as judged by measuring ear thickness and weight, and histological analysis. Topical administration of AC also reduced the expression of pro-inflammatory cytokines such as TNF-¥á, IL-1¥â, and IL-6 in TPA-stimulated mouse ears. Pretreatment with AC suppressed TNF-¥á-induced ICAM-I expression and subsequent monocyte adhesiveness in human keratinocyte cell line HaCaT. In addition, AC significantly decreased intracellular reactive oxygen species (ROS) generation as well as mitogen-activated protein kinase (MAPK) activation in TNF-¥á-stimulated HaCaT cells. AC and its constituent cyanidin 3-glucoside also attenuated TNF-¥á-induced IKK activation, I¥êB degradation, p65 phosphorylation/nuclear translocation, and p65 DNA binding activity in HaCaT cells. Overall, our results indicate that AC exerts anti-inflammatory activities by inhibiting expression of pro-inflammatory mediators in vitro and in vivo possibly through suppression of ROS-MAPK-NF-¥êB signaling pathways. Therefore, AC may be developed as a therapeutic agent to treat various inflammatory skin diseases.
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KEYWORD
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Aronia melanocarpa, cytokines, edema, ICAM-1, inflammation, keratinocyte, TNF-¥á, TPA
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